Structural-functional analysis of engineered protein-nanoparticle assemblies using graphene microelectrodes† †Electronic supplementary information (ESI) available. See DOI: 10.1039/c7sc01565h Click here for additional data file.

نویسندگان

  • Jinglei Ping
  • Katherine W. Pulsipher
  • Ramya Vishnubhotla
  • Jose A. Villegas
  • Tacey L. Hicks
  • Stephanie Honig
  • Jeffery G. Saven
  • Ivan J. Dmochowski
  • A. T. Charlie Johnson
چکیده

The characterization of protein-nanoparticle assemblies in solution remains a challenge. We demonstrate a technique based on a graphene microelectrode for structural-functional analysis of model systems composed of nanoparticles enclosed in open-pore and closed-pore ferritin molecules. The method readily resolves the difference in accessibility of the enclosed nanoparticle for charge transfer and offers the prospect for quantitative analysis of pore-mediated transport, while shedding light on the spatial orientation of the protein subunits on the nanoparticle surface, faster and with higher sensitivity than conventional catalysis methods.

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Structural-functional analysis of engineered protein-nanoparticle assemblies using graphene microelectrodes.

The characterization of protein-nanoparticle assemblies in solution remains a challenge. We demonstrate a technique based on a graphene microelectrode for structural-functional analysis of model systems composed of nanoparticles enclosed in open-pore and closed-pore ferritin molecules. The method readily resolves the difference in accessibility of the enclosed nanoparticle for charge transfer a...

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عنوان ژورنال:

دوره 8  شماره 

صفحات  -

تاریخ انتشار 2017